The £52m striker, who made a late appearance as a substitute in yesterday’s 5-2 win over Benfica, is one of 11 changes made by Arsene Wenger.
Laurent Koscielny, Mohamed Elneny and Nacho Monreal line up in a three-man defence in front of Petr Cech.
Hector Bellerin and Alex Oxlade-Chamberlain are at wing-back while Aaron Ramsey and Joseph Willock start in midfield.
Lacazette receives support in attack from Mesut Ozil and Danny Welbeck.
Sevilla can challenge Arsenal for the Emirates Cup title after beating RB Leipzig 1-0 yesterday but they need to win by a hefty margin.
Arsenal: Cech; Koscielny, Elneny, Monreal; Bellerin, Ramsey, Willock, Oxlade-Chamberlain; Ozil, Welbeck, Lacazette
Subs: Martinez, Mertesacker, Holding, Kolasinac, Bramall, Maitland-Niles, Xhaka, Nelson, Iwobi, Walcott, Giroud
Sevilla: David Soria; Mercado, Pareja, Carrico, Escudero; NZonzi, Krohn-Dehli, Banega; Correa, Nolito, Ben Yedder