Defender Ben Chilwell has withdrawn from the England Under-21 squad to face Scotland and Andorra.
The Leicester left-back has returned to his club for treatment on a knee injury and while it is not believed to be serious he will not feature in the Young Lions' latest Euro 2019 qualifiers.
Arsenal's Ainsley Maitland-Niles (knock) and Everton defender Jonjoe Kenny (back) missed training at St George's Park on Wednesday as they have treatment for minor knocks but remain in contention to face Scotland on Friday.
Boss Aidy Boothroyd said: "It's not serious. Ben came in, we scanned him but we decided it would be best he goes back to his club. We've got a couple more to assess. We have Ainsley and Jonjoe, they are both going for scans. They were only precautionary so we'll know where we are with them.
"We've got a couple [of players] on stand-by.''
Boothroyd's side welcome Scotland to Middlesbrough's Riverside Stadium with England joint top of Group Four and Scotland a point behind in third place.
The Young Lions, who were beaten in the Euro 2017 semifinals on penalties by Germany in the summer, then travel to rock bottom Andorra for a game on October 10.
They have already drawn 1-1 with the Netherlands and beaten Latvia 3-0.